Coding

Part:BBa_K4451008:Design

Designed by: Brooks J Rady   Group: iGEM22_Sheffield   (2022-09-30)


T4 Alc


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 7
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 7
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 413
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 7
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 7
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

References

Kashlev, M., Nudler, E., Goldfarb, A., White, T., and Kutter, E. Bacteriophage T4 Alc protein: a transcription termination factor sensing local modification of DNA. Cell 75: 147–154 (1993).

Roucourt, B. and Lavigne, R. The role of interactions between phage and bacterial proteins within the infected cell: a diverse and puzzling interactome. Environmental Microbiology 11(11), 2789-2805 (2009).